Coding

Part:BBa_K4361312:Design

Designed by: Lars van den Biggelaar   Group: iGEM22_TUDelft   (2022-09-19)


BlcR L66I


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589


Design Notes

-Ensure that the mutant complies with BioBrick standards


Source

-Part:BBa_K4361100 after mutation with Part:BBa_K4361218 and Part:BBa_K4361221

References